Composite
Part:BBa_K5301020:Design
Designed by: Jiaxin Guan Group: iGEM24_BNU-China (2024-09-24)
SnCSdT expression pathway (T7)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1081
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1223
Illegal PstI site found at 1081 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 263
Illegal BglII site found at 635 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1081
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1081
Illegal AgeI site found at 160
Illegal AgeI site found at 920 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lac operator helps it to express in T7 system.
Source
It's genetically engineered to produce large nanodiscs more conveniently .